عنوان مقاله:

نویسندگان:

baradaranrv@mums.ac.ir برادران رحیمی، وحید رضا عسکری

Background Sepsis causes organ dysfunctions via elevation of oxidative stress and inflammation. Lipopolysaccharide (LPS) is the major surface molecule of most gram-negative bacteria and routinely used as a sepsis model in investigation studies. Crocin is an active compound of saffron which has different pharmacological properties such as anti-oxidant and anti-inflammatory. In this research, the protective effect of crocin was evaluated against LPS-induced toxicity in the embryonic cardiomyocyte cell line (H9c2). Methods The cells were pre-treated with different concentration of crocin (10, 20 and 40 μM) for 24 h, and then LPS was added (10 μg/ml) for another 24 h. Afterward, the percentage of cell viability and the levels of inflammatory cytokines (TNF-α, PGE2, IL-, and IL-6), gene expression levels (TNF-α, COX-2, IL-, IL-6, and iNOS), and the level of nitric oxide (NO) and thiol were measured. Results Our results showed that LPS reduced the cell viability and and thiol content, while increased the levels of cytokines, gene expression, nitric oxide in H9c2 cell. Crocin attenuated the LPS-induced toxicity in H9c2 cells via reducing the levels of inflammatory factors (TNF-α, PGE2, IL-b, and IL-6, p <0.001), gene expression (TNF-α, COX-2, IL-b, IL-6, and iNOS, p <0.001), and NO (p <0.001), whereas increased the level of thiol content (p <0.001). Conclusion The observed results revealed that crocin has preventive effects on the LPS induced sepsis and its cardiac toxicity in an in-vitro model. Probably, these findings are related to anti-inflammatory and anti-oxidant properties of crocin. However, performing further animal studies are necessary to support the therapeutic effects of crocin in septic shock cardiac dysfunction.

Sepsis, Lipopolysaccharide, Inflammation, H9c2, cardio-toxicity

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برادران رحیمی، baradaranrv@mums.ac.ir، رضا عسکری، وحید، 1400، Crocin Protects Cardiomyocytes against LPS-Induced Inflammation، سیزدهمین کنگره قلب و عروق خاورمیانه و ششمین همایش مداخلات پیچیده قلبی، http://conf.sums.ac.ir/fa/archive_index.php?c=HN-mecc13&aid=1512537