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نویسندگان:

حمیدرضا گلیان، ندا اصل استیاری، احسان متی پور، امین متی پور، آرمین امیری، شهاب ملکی

Background: Umbilical cord stem cells have numerous therapeutic applications due to their differentiation potential, accessibility, and risk-free procurement. This study utilized the umbilical cord as the primary source of stem cell production, and mouse lung extract, rich in hematopoietic growth factors, was employed to stimulate differentiation into blood cells. Methods: In this fundamental, applied, and practical research, stem cells were initially isolated from the umbilical cord via enzymatic digestion and cultured under suitable conditions. Four experimental groups were studied: MGF50% and MGF75% groups, exposed to 50% and 75% concentrations of mouse lung extract for seven days, the sham group, cultured for seven days without lung extract exposure, and the control group comprising normal mouse blood cells. All cultured groups were evaluated using the alkaline phosphatase kit for stem cells, followed by differential blood cell count and hematopoietic factor measurement. Data were analyzed using one-factor analysis of variance. Results: Significant changes were observed in the MGF75% group compared to control and sham groups, indicating differentiation of stem cells into erythroid and myeloid lineages. Also, by evaluating the response of the immune system, no acute response was observed. No morphological and receptor changes were observed in hematopoietic stem cell lines or blood progenitors. Conclusion: The growth factors present in lung extract can stimulate the differentiation of umbilical cord stem cells into blood cells. The results of the above study can be used as a new strategy in the field of supportive treatment of blood cancer patients. It has great economic potential and can be used as a therapeutic candidate without immune system complications or tissue symptoms.

Umbilical cord, Stem cell, Lung extract, Erythroid differentiation, Myeloid differentiation

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گلیان، حمیدرضا، اصل استیاری، ندا، متی پور، احسان، متی پور، امین، امیری، آرمین، ملکی، شهاب، 1404، عصاره ریه به عنوان یک عامل محرک برای خون سازی: گسترش کاربرد سلول های بنیادی بند ناف؛ درمان حمایتی لوسمی، کنگره بین المللی زیست پزشکی سرطان و سلولهای بنیادی، http://conf.sums.ac.ir/fa/archive_index.php?c=HN-icsbc1&aid=1514166