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بهینا بازقندی، فتانه فاطمی، احسان صبوری

Background: Alpha-N-acetylgalactosaminidase, commonly known as Nagalase, is an enzyme encoded by the NAGA gene, highly expressed in liver cells. Nagalase breaks down specific sugar chains on sphingolipids and glycoproteins, contributing to lysosomal storage. Elevated levels of Nagalase are frequently observed in cancer patients, though there is no structural difference between naturally occurring and cancer-associated Nagalase. In cancer, Nagalase shifts from a lysosomal (acidic pH) to an extracellular (neutral pH) function, which may impact its activity. Nagalase levels are correlated with cancer progression and metastasis, suggesting its potential as a biomarker for early diagnosis, prognosis, and monitoring. This study aims to investigate the relationship between Nagalase expression and cancer cell migration and metastasis across different cell lines. Methods: We prepared 10 standard cancer cell lines (MCF7, MDA-MB231, HepG2, HeLa, SKBR3, EPG, A780, SC, HUH, and CACO2) and performed RNA extraction and cDNA synthesis. Using qRT-PCR, we assessed NAGA gene expression relative to the beta-actin gene. Cell migration was evaluated via the scratch assay, while metastatic potential was analyzed using the Matrigel chamber assay. Statistical correlation tests were conducted at a significance level of 0.05 using GraphPad software. Results: Results indicate a significant relationship between NAGA expression levels and the migration and metastasis abilities of the cancer cell lines. While CT value changes aligned with increased migratory and metastatic abilities, these changes did not reach statistical significance (p > 0.05). Conclusion: While a relationship between NAGA gene expression and cancer cell migration and metastasis is suggested, the small sample size and lack of repetition may limit statistical significance. These findings are consistent with previous studies and suggest that increasing sample size and further studies in cancer patients may yield more robust insights into the role of Nagalase in cancer progression.

Alpha-N-acetylgalactosaminidase, cancer, extracellular matrix

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بازقندی، بهینا، فاطمی، فتانه، صبوری، احسان، 1404، بررسی بیان ژن آلفا-N-استیل گالاکتوزامینیداز و ارتباط آن با مهاجرت و متاستاز در رده های سلولی سرطانی، کنگره بین المللی زیست پزشکی سرطان و سلولهای بنیادی، http://conf.sums.ac.ir/fa/archive_index.php?c=HN-icsbc1&aid=1514202